We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Visualization of early embryos of the butterfly Bicyclus anynana.
- Authors
Jarod Masci; Antónia Monteiro
- Abstract
We report on the first attempts, using both light and fluorescence microscopy, to visualize the developing embryo of the butterfly Bicyclus anynana. We developed a new protocol that enabled the clear visualization of the internal egg structures in early embryogenesis (1–24 h after egg laying). Dechorionation was followed by fixation and physical dissection of the external egg structures. Observations of embryonic and extra-embryonic cells were made using a Hoechst nuclear stain that fluoresces in the blue spectrum when bound to DNA and excited with ultraviolet (UV) light under a fluorescence microscope. Preliminary data on the developmental rate of the early embryo are also presented.
- Publication
Zygote, 2005, Vol 13, Issue 2, p139
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/s0967199405003163