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- Title
Advances in the production of human therapeutic proteins in yeasts and filamentous fungi.
- Authors
Gerngross, Tillman U.
- Abstract
Yeast and fungal protein expression systems are used for the production of many industrially relevant enzymes, and are widely used by the research community to produce proteins that cannot be actively expressed in Escherichia coli or require glycosylation for proper folding and biological activity. However, for the production of therapeutic glycoproteins intended for use in humans, yeasts have been less useful because of their inability to modify proteins with human glycosylation structures. Yeast glycosylation is of the high-mannose type, which confers a short in vivo half-life to the protein and may render it less efficacious or even immunogenic. Several ways of humanizing yeast-derived glycoproteins have been tried, including enzymatically modifying proteins in vitro and modulating host glycosylation pathways in vivo. Recent advances in the glycoengineering of yeasts and the expression of therapeutic glycoproteins in humanized yeasts have shown significant promise, and are challenging the current dominance of therapeutic protein production based on mammalian cell culture.
- Subjects
PROTEINS; YEAST; FILAMENTOUS fungi; ESCHERICHIA coli; GLYCOPROTEINS; GLYCOSYLATION
- Publication
Nature Biotechnology, 2004, Vol 22, Issue 11, p1409
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt1028