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- Title
Anti-Acanthamoeba Activity of Contact Lens Disinfecting Solutions against Acanthamoeba Cysts.
- Authors
Abd Ghani, Mohamed Kamel; Santokh Singh, Kirat Kaur; Nordin, Anisah; Suboh, Yusof; Rahim, Noraina Ab; Ahmad, Norazah
- Abstract
Introduction: Acanthamoeba keratitis incidence amongst contact lens wearers is increasing. The effectiveness of commercially available contact lens disinfecting solutions as anti-Acanthamoeba is doubtful. Objective: This study investigates the anti-Acanthamoeba activity of Complete® and Revita Lens Ocutec™ contact lens disinfecting solutions against 4 clinical isolates of Acanthamoeba and to determine its efficacy based on the soaking time recommended by the manufacturers and soaking times of 4 hours, 6 hours and 8 hours. Materials and methods: Cyst suspensions were prepared using Acanthamoeba cysts grown on Non-Nutrient Agar (NNA) for 11 days at 30°C (± 2°C). Two brands of commercially available contact lens disinfecting solutions were used to determine its effectiveness as anti-Acanthamoeba agents. Cyst suspensions of each strain were tested against each contact lens disinfecting solution based on the manufacturers recommended soaking time, 4 hours, 6 hours and 8 hours respectively. After the soaking time, 100 pi cyst suspension of each strain was cultured onto NNA plates seeded with E. coli at 30°C (± 2°C) and observed daily for 14 days under an inverted microscope to detect the presence of trophozoites. Results: Complete® and Revita Lens Ocutec™ were ineffective at inactivating the Acanthamoeba cysts within the testing times. Revita Lens OcutecTM was ineffective against Acanthamoeba cysts despite claims as having anti-Acanthamoeba property. Most commercially available contact lens disinfecting solutions are ineffective as anW-Acanthamoeba agents.
- Subjects
MALAYSIA; ACANTHAMOEBA keratitis; CONTACT lenses; CULTURE media (Biology); CULTURES (Biology); CYSTS (Pathology); OPHTHALMIC drugs; OPTOMETRY; PROTOZOA; DATA analysis software; DESCRIPTIVE statistics; IN vitro studies; DISEASE risk factors
- Publication
International Medical Journal, 2016, Vol 23, Issue 6, p720
- ISSN
1341-2051
- Publication type
Article