We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Primary cilium-dependent mechanosensing is mediated by adenylyl cyclase 6 and cyclic AMP in bone cells.
- Authors
Kwon, Ronald Y.; Temiyasathit, Sara; Tummala, Padmaja; Quah, Clarence C.; Jacobs, Christopher R.
- Abstract
Primary cilia are chemosensing and mechanosensing organelles that regulate remarkably diverse processes in a variety of cells. We previously showed that primary cilia play a role in mediating mechanosensing in bone cells through an unknown mechanism that does not involve extracellular Ca2+-dependent intracellular Ca2+ release, which has been implicated in all other cells that transduce mechanical signals via the cilium. Here, we identify a molecular mechanism linking primary cilia and bone cell mechanotransduction that involves adenylyl cyclase 6 (AC6) and cAMP. Intracellular cAMP was quantified in MLO-Y4 cells exposed to dynamic flow, and AC6 and primary cilia were inhibited using RNA interference. When exposed to flow, cells rapidly (<2 min) and transiently decreased cAMP production in a primary ciliumdependent manner. RT-PCR revealed differential expression of the membrane-bound isoforms of adenylyl cyclase, while immunostaining revealed one, AC6, preferentially localized to the cilium. Further studies showed that decreases in cAMP in response to flow were dependent on AC6 and Gd3+-sensitive channels but not intracellular Ca2+ release and that this response mediated flow-induced COX-2 gene expression. The signaling events identified provide important details of a novel early mechanosensing mechanism in bone and advances our understanding of how signal transduction occurs at the primary cilium.
- Subjects
OSTEOCYTES; OSTEOPOROSIS; CELLS; OVUM; BONE cells; ADENYLATE cyclase
- Publication
FASEB Journal, 2010, Vol 24, Issue 8, p2859
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.09-148007