We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phosphorus magnetic resonance spectroscopy 2 h after perinatal cerebral hypoxia-ischemia prognosticates outcome in the newborn piglet.
- Authors
Cady, Ernest B.; Iwata, Osuke; Bainbridge, Alan; Wyatt, John S.; Robertson, Nicola J.
- Abstract
Phosphorus magnetic resonance spectroscopy (31P MRS) often reveals apparently normal brain metabolism in the first hours after intrapartum hypoxia-ischemia (HI) at a time when conventional clinical assessment of injury severity is problematic. We aimed to elucidate very-early, injury-severity biomarkers. Twenty-seven newborn piglets underwent cerebral HI: 31P-MRS measures ∼2 h after HI were compared between injury groups defined by secondary-energy-failure severity as quantified by the minimum nucleotide triphosphate (NTP) observed after 6 h. For severe and moderate injury versus baseline, [Pi]/[total exchangeable high-energy phosphate pool (EPP)] was increased ( p < 0.001 and < 0.02, respectively), and [NTP]/[EPP] decreased ( p < 0.03 and < 0.006, respectively): severe-injury [Pi]/[EPP] was also increased versus mild injury ( p < 0.04). Mild-injury [phosphocreatine]/[EPP] was increased ( p < 0.004). Severe-injury intracellular pH was alkaline versus baseline ( p < 0.002). For severe and moderate injury [total Mg]/[ATP] ( p < 0.0002 and < 0.02, respectively) and [free Mg] ( p < 0.0001 and < 0.02, respectively) were increased versus baseline. [Pi]/[EPP], [phosphocreatine]/[Pi] and [NTP]/[EPP] correlated linearly with injury severity ( p < 0.005, < 0.005 and < 0.02, respectively). Increased [Pi]/[EPP], intracellular pH and intracellular Mg ∼2 h after intrapartum HI may prognosticate severe injury, whereas increased [phosphocreatine]/[EPP] may suggest mild damage. In vivo31P MRS may have potential to provide very-early prognosis in neonatal encephalopathy.
- Subjects
HYPOXEMIA; ISCHEMIA; PIGLETS; MAGNETIC resonance imaging; BIOMARKERS; NEUROCHEMISTRY
- Publication
Journal of Neurochemistry, 2008, Vol 107, Issue 4, p1027
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2008.05662.x