We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Anti-diabetic activity-guided screening of aqueous-ethanol Moringa oleifera extracts and fractions: Identification of marker compounds.
- Authors
Irfan, Hafiz Muhammad; Asmawi, Mohd Zaini; Khan, Nurzalina Abdul Karim; Sadikun, Amirin; Mordi, Mohd Nizam
- Abstract
Purpose: To explore the anti-diabetic effects of Moringa oleifera extracts and fractions, and to identify their active/marker compounds. Methods: Five different aqueous ethanol extracts (95, 75, 50, 25%v/v and 100% water) of Moringa oleifera were given orally to normal rats to assess their hypoglycemic activities and effect on intraperitoneal glucose tolerance test (IPGTT) data. Rats with streptozotocin-induced diabetes were used to assess acute and sub-chronic anti-hyperglycemic activities. The most active extract was further subjected to liquid-liquid fractionation into hexane, chloroform, ethyl acetate, butanol, and water; these fractions were screened for anti-diabetic activities. The most active extract, and fractions thereof, were then subjected to qualitative and quantitative phytochemical analysis. Standardization was achieved via thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC), and used to identify marker compounds. Results: Of all the extracts and fractions, 95% (v/v) ethanol extract (at 1,000 mg/kg) and the butanol fraction thereof (at 500 mg/kg) were the most active, reducing blood glucose concentration after onetime (acute) administration to diabetic rats (p < 0.01). No significant hypoglycemic activity was apparent, and the materials had no effect on IPGTT performance by normal rats. TLC and HPLC identified quercetin 3-β-D-glucoside, kaempferol-3-O-glucoside, and cryptochlorogenic acid. Conclusion: An M. oleifera leaf extract exhibited anti-hyperglycaemic activity in diabetic rats only. This effect was likely attributable to cryptochlorogenic acid, quercetin 3-β-D-glucoside, and kaempferol 3-Oglucoside.
- Subjects
MORINGA oleifera; BIOMARKERS; HYPOGLYCEMIC agents; PEOPLE with diabetes; HIGH performance liquid chromatography; STREPTOZOTOCIN; GLUCOSE tolerance tests; LABORATORY rats; PHYSIOLOGY
- Publication
Tropical Journal of Pharmaceutical Research, 2017, Vol 16, Issue 3, p543
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v16i3.7