Investigation of the biological activities of Phu Quoc Sim fruits Rhodomyrtus tomentosa (aiton) hassk.

Vo Thanh Sang; Ngo Dai Hung; Le Phuong Uyen; Ngo Dai Nghiep; Kim Se-Kwon

Background: Rhodomyrtus tomentosa (Aiton) Hassk., locally known as Sim, is a flowering plant belonging to the family Myrtacea. It have been used in traditional Vietnamese medicine for a long time for treatment of diarrhoea, dysentery, gynaecopathy, stomachache, and wound healing. In this study, the potential bioactivities of ethanol extract of Sim fruits due to scavenging free radicals, suppressing pro-inflammatory mediator production, and inhibiting histamine release were investigated in vitro. Material and Methods: Sim fruits were obtained from from Duong Dong Town, Phu Quoc district, Kien Giang province, Viet Nam. The crude extract was prepared with Sim fruits in ethanol 80%. The free radical scavenging activity was conducted via DPPH and ABTS assay. Nitric oxide (NO) production was measured by colorimetric assay and inducible nitric oxide synthase (iNOS) expression was conducted by Western blot. Histamine release was measured by spectrofluorometric assay. Results: Ethanol extract Sim fruits (EESF) was found to be able to scavenge DPPH and ABTS+ radicals with IC50 values of 94 ± 12 and 83 ± 9 µg/ml, respectively. Moreover, NO production and iNOS protein expression were suppressed by EESF treatment at a dose-dependent manner in lipopolysaccharide-stimulated RAW 264.7 cells. In addition, EESF was effective in reducing histamine release from calcium ionophore-activated RBL-2H3 mast cells. Conclusion: These results indicated the potential health benefit effects of Sim fruit due to its biological activities. These evidences supported it as a promising biomaterial for alternative medicine.

MYRTACEAE; DIARRHEA; DYSENTERY; TREATMENT of abdominal pain; NITRIC-oxide synthases

EurAsian Journal of Biosciences, 2019, Vol 13, Issue 1, p49

1307-9867

Academic Journal