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- Title
Inhibitory effect of Samul-tang on retinal neovascularization in oxygen-induced retinopathy.
- Authors
Yun Mi Lee; Chan-Sik Kim; Kyuhyung Jo; Eun Jin Sohn; Jin Sook Kim; Junghyun Kim
- Abstract
Background: Retinal neovascularization is a common cause of vision loss in proliferative diabetic retinopathy, retinopathy of prematurity and age-related macular degeneration. Samul-tang (SMT) is a widely used traditional herbal medicine in East Asia and is also known as Shimotsu-to in Japanese and Si-Wu decoction in Chinese. This study was designed to evaluate the inhibitory effect of SMT on retinal pathogenic angiogenesis in a mouse model of oxygen-induced retinopathy (OIR). Method: The mice were exposed to a 75 % concentration of oxygen for five days, starting on postnatal day 7 (P7-P12). The mice were then exposed to room air and were intraperitoneally injected with SMT (10 mg/kg or 50 mg/kg) once per day for five days (P12-P16). On P17, we measured retinal neovascularization and evaluated both the expression of angiogenesis-related proteins and changes in the gene expression level in the mRNA. Results: SMT reduced the area of the central retina and reduced retinal neovascularization in OIR mice. The protein array revealed that SMT reduced the level of SDF-1 protein expression. Quantitative real-time PCR revealed that the HIF-1α, SDF-1, CXCR4 and VEGF mRNA levels in the retinas of OIR mice were elevated compared with those of normal control mice. However, SMT decreased the levels of HIF-1α, SDF-1, CXCR4 and VEGF mRNA in OIR mice. Conclusion: We are the first to elucidate that SMT inhibits the retinal pathogenic angiogenesis induced by ischemic retinopathy in OIR mice. SMT significantly inhibited retinal neovascularization by downregulating HIF-1α, SDF-1, CXCR4 and VEGF. Based on the results of our study, SMT could be a useful herbal medicine for treating ischemic retinopathy.
- Subjects
ANGIOGRAPHY; ANIMAL experimentation; DIABETIC retinopathy; HIGH performance liquid chromatography; MEDICINAL plants; ASIAN medicine; MICE; MICROSCOPY; POLYMERASE chain reaction; RESEARCH funding; RETINAL degeneration; RETROLENTAL fibroplasia; REVERSE transcriptase polymerase chain reaction; PROTEIN microarrays; PATHOLOGIC neovascularization; IN vitro studies
- Publication
BMC Complementary & Alternative Medicine, 2015, Vol 15, Issue 1, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-015-0800-7