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- Title
Endogenous feline leukemia virus long terminal repeat integration site diversity is highly variable in related and unrelated domestic cats.
- Authors
Chiu, Elliott S.; McDonald, Coby A.; Gagne, Roderick B.; Dunkleberger, Henry; Moxcey, Matthew; VandeWoude, Sue
- Abstract
Endogenous retroviruses (ERV) are indicators of vertebrate evolutionary history and play important roles as homeostatic regulators. ERV long terminal repeat (LTR) elements may act as cis-activating promoters or trans-activating enhancer elements modifying gene transcription distant from LTR insertion sites. We previously documented that endogenous feline leukemia virus (FeLV)-LTR copy number variation in individual cats tracks inversely with susceptibility to virulent FeLV disease. To evaluate FeLV-LTR insertion characteristics, we assessed enFeLV-LTR integration site diversity in 20 cats from three genetically distinct populations using a baited linker-mediated PCR approach. We documented 765 individual integration sites unequally represented among individuals. Only three LTR integration sites were shared among all individuals, while 412 sites were unique to a single individual. When primary fibroblast cultures were challenged with exogenous FeLV, we found significantly increased expression of both exogenous and endogenous FeLV orthologs, supporting previous findings of potential exFeLV-enFeLV interactions; however, viral challenge did not elicit transcriptional changes in genes associated with the vast majority of integration sites. This study assesses FeLV-LTR integration sites in individual animals, providing unique transposome genotypes. Further, we document substantial individual variation in LTR integration site locations, even in a highly inbred population, and provide a framework for understanding potential endogenous retroviral element position influence on host gene transcription.
- Subjects
FELINE leukemia virus; CATS; CAT diseases; ENDOGENOUS retroviruses
- Publication
Retrovirology, 2024, Vol 21, Issue 1, p1
- ISSN
1742-4690
- Publication type
Article
- DOI
10.1186/s12977-024-00635-0