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- Title
Detection of miRNA in Live Cells by Using Templated Ru<sup>II</sup>-Catalyzed Unmasking of a Fluorophore.
- Authors
Sadhu, Kalyan K.; Winssinger, Nicolas
- Abstract
Reactions templated by cellular nucleic acids are attractive for nucleic acid sensing or responsive systems. Herein we report the use of a photocatalyzed reductive cleavage of an immolative linker to unmask a rhodamine fluorophore, and its application to miRNA imaging. The reaction was found to proceed with a very high turnover (>4000) and provided reliable detection down to 5 p M of template by using γ-serine-modified peptide nucleic acid (PNA) probes. The reaction was used for the selective detection of miR-21 in BT474 cells and miR-31 in HeLa cells following irradiation for 30 min. The probes were introduced by using reversible permeation with streptolysin-O (SLO) or a transfection technique.
- Subjects
MICRORNA; RNA; CELLS; FLUOROPHORES; CHEMICALS
- Publication
Chemistry - A European Journal, 2013, Vol 19, Issue 25, p8182
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201300060