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- Title
Reading of Protein Surfaces in the Native State at Micromolar Concentrations by a Chirogenetic Porphyrin Probe.
- Authors
Mineo, Placido; Micali, Norberto; Villari, Valentina; Donato, Maria Grazia; Scamporrino, Emilio
- Abstract
The recognition of some globular proteins was carried out in aqueous solution, at micromolar concentrations, by using an uncharged symmetrical cobalt-porphyrin (Co-P). By means of UV/Vis, induced circular dichroism, and fluorescence spectroscopy techniques, it was ascertained that the interactions between specific amino acid residues and Co-P occurred on the protein surface. In particular, spectroscopic evidence showed the formation of supramolecular complexes without disruption of the native structure of the proteins and, furthermore, that signal changes were characteristic of each Co-P/protein system, so that they could be used as a highly sensitive analytical tool for protein recognition. The relative association constants were proportional to the protein molecular masses (and then to the number of amino acid residues).
- Publication
Chemistry - A European Journal, 2012, Vol 18, Issue 39, p12452
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201200784