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- Title
In vivo DTI assessment of hepatic ischemia reperfusion injury in an experimental rat model.
- Authors
Cheung, Jerry S.; Fan, Shu Juan; Chow, April M.; Hui, Edward S.; Wu, Ed X.
- Abstract
Purpose To investigate hepatic ischemia reperfusion injury (IRI) using diffusion tensor imaging (DTI). Materials and Methods Ten Sprague-Dawley rats were scanned at 7 Tesla (T) with DTI using b-value of 1000 s/mm2 and 6 gradient directions before, 2 h, and 1 day after 30-min total hepatic IRI. Apparent diffusion coefficient or mean diffusivity (MD), directional diffusivities and fractional anisotropy (FA) were measured. Seven of the animals were also examined with spin-echo echo-planar diffusion-weighted imaging (DWI) with seven b-values up to 2000 s/mm2 to estimate the true diffusion coefficient (D), blood pseudodiffusion coefficient (D*), and perfusion fraction (f) using a bi-compartmental model. Results MD 2 h after IRI (0.77 ± 0.07 × 10−3 mm2/s) was significantly lower ( P < 0.01) than that before (1.03 ± 0.07 × 10−3 mm2/s) and 1 day after IRI (1.01 ± 0.05 × 10−3 mm2/s). Meanwhile, FA 2 h after IRI (0.33 ± 0.03) was significantly higher ( P < 0.01) than that before (0.21 ± 0.02) and 1 day after IRI (0.20 ± 0.02). The bi-compartmental model analysis revealed the transient decrease in D, D* and f 2 h after IRI. Liver histology showed the multifocal cell swelling 3 h after IRI and widespread cell necrosis/apoptosis 1 day after IRI. Sinusoidal narrowing and congestion of erythrocytes were also observed 3 h and 1 day after IRI. Conclusion DTI can characterize hepatic IRI by detecting the transient change in both MD and FA. J. Magn. Reson. Imaging 2009;30:890-895. © 2009 Wiley-Liss, Inc.
- Publication
Journal of Magnetic Resonance Imaging, 2009, Vol 30, Issue 4, p890
- ISSN
1053-1807
- Publication type
Article
- DOI
10.1002/jmri.21917