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- Title
Pro-inflammatory cellular immune response in Behçet’s disease.
- Authors
Kulaber, Ayla; Tugal-Tutkun, Ilknur; Yentür, Sibel P.; Akman-Demir, Gülşen; Kaneko, Fumio; Gül, Ahmet; Saruhan-Direskeneli, Güher
- Abstract
Several proteins are investigated as candidate auto-antigens in the pathogenesis of Behçet’s disease (BD). This study aimed to screen the cellular responses to auto-antigen (αB-crystallin, αBC), and microorganism ( Streptococcus sangius KTH-1 BES-1 protein) derived peptides as well as to isopentenyl pyrophosphate (IPP). Peripheral blood mononuclear cells (PBMC) from 22 BD patients and 22 healthy controls (HC) were stimulated in vitro with αBC, BES-1 peptides, IPP and PPD and induced proliferation as well as the secreted interleukin (IL)-12 and interferon (IFN)-γ production levels were measured. We did not observe any significant difference in cellular proliferation between BD patients and HC. Induction of IL-12 secretion with αBC was stronger in BD patients ( P = 0.04) and in the disease subgroup with uveitis ( P = 0.027) compared the HC. When responses to PPD were compared, proliferation of PMBC was lower ( P = 0.03), whereas IL-12 secretion was higher in BD ( P = 0.04) as well as in patients under colchicum treatment ( P = 0.04) and with vascular involvement ( P = 0.006) compared to HC. BES-1373–385 peptide induced also higher IL-12 productions by PBMC of BD patients ( P = 0.017) and of patients with uveitis ( P = 0.013). Finally, IPP stimulated higher IL-12 secretions from PBMC in BD patients ( P = 0.035) and in patients with ( P = 0.02) or without ( P = 0.017) uveitis or arthritis ( P = 0.04), under colchicum treatment ( P = 0.01) or not receiving any immunosuppressive treatment ( P = 0.007) compared to HC. These results suggest a more prominent pro-inflammatory cytokine secretion from PBMC in BD patients compared to HC in response to various antigens including αBC protein, BES-1373–385, IPP and PPD.
- Subjects
IMMUNE response; BEHCET'S disease; PEPTIDES; INFLAMMATION; IMMUNOGLOBULINS; ANTINEOPLASTIC agents; ANTIVIRAL agents
- Publication
Rheumatology International, 2007, Vol 27, Issue 12, p1113
- ISSN
0172-8172
- Publication type
Article
- DOI
10.1007/s00296-007-0367-9