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- Title
Sexual introgression of the late blight resistance gene Rpi-blb3 from a Mexican wild diploid species Solanum pinnatisectum Dunal into potato varieties.
- Authors
Sanetomo, Rena; Habe, Ippei; Hosaka, Kazuyoshi
- Abstract
Solanum pinnatisectum Dunal (2n = 2x = 24) is a tuber-bearing Mexican wild species that has an extremely high level of resistance to late blight (Phytophthora infestans). However, this species is distantly related and isolated from cultivated potato species by a strict sexual reproduction barrier. Previously, we successfully obtained triploid interspecific hybrids between chromosome-doubled S. pinnatisectum and a South American diploid clone carrying the S locus inhibitor gene (Sli). In this study, chromosome doubling of triploid hybrids was done to produce hexaploids used as the recurrent and donor parents in the successive backcrossing scheme. In a BC2 population consisting of 198 genotypes, P. infestans was inoculated. Ninety-six genotypes were resistant, and 102 were susceptible. The presence of the Rpi-blb3-specific marker band perfectly matched the resistance phenotype, verifying that the Rpi-blb3 gene controlled resistance. BC1 and BC2 progenies were relatively easy to reciprocally cross with cultivars. Therefore, the Rpi-blb3 gene derived from S. pinnatisectum could be used in practical breeding to confer a high level of late blight resistance to potato varieties with the aid of the gene-specific marker.
- Subjects
LATE blight of potato; INTROGRESSION (Genetics); DIPLOIDY; PLANT species; POTATO genetics
- Publication
Molecular Breeding, 2019, Vol 39, Issue 1, p1
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-018-0924-9