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- Title
Identification of a specific molecular marker for the rice blast-resistant gene Pigm and molecular breeding of thermo-sensitive genic male sterile leaf-color marker lines.
- Authors
Dai, Xiaojun; He, Chao; Zhou, Liang; Liang, Manzhong; Fu, XiaoChun; Qin, Peng; Yang, Yuanzhu; Chen, Liangbi
- Abstract
Rice blast is a damaging disease caused by Magnaportheoryzae. Marker-assisted selection of blast resistance genes could help develop cultivars with blast resistance. Pigm is a broad-spectrum blast-resistant gene. However, few rice resources contain Pigm. In this study, the Pigm gene donor Gumei4 (GM4) was investigated. By analyzing different regions of Pigm sequences, we found that marker G8900 was a specific molecular marker of Pigm gene in GM4. Correlation analysis between molecular marker detection and identification of rice blast disease nursery revealed that G8900 could be used in marker-assisted selection (MAS) of Pigm. Furthermore, we introduced Pigm gene into the KT27S line (a blast-susceptible yellow-green-leaf-color mutant) in G8900-assisted breeding and identified three new yellow-green-leaf-color marker lines that are resistant to blast. The agronomic and economic traits of the three new lines are similar to those of their parental lines. The identification and application of Pigm-specific molecular marker in breeding of yellow-green-leaf-color marker line could play an important role in the production of disease-resistant hybrid rice.
- Subjects
GENETIC markers in plants; RICE blast disease; PYRICULARIA grisea; PLANT breeding; RICE disease &; pest resistance
- Publication
Molecular Breeding, 2018, Vol 38, Issue 6, p1
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-018-0821-2