We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Differentially expressed proteins during an incompatible interaction between common bean and the fungus <i>Pseudocercospora griseola</i>.
- Authors
Borges, Leandro Luiz; Santana, Fernanda Abreu; Castro, Isabel Samila Lima; Arruda, Klever Márcio Antunes; de Oliveira Ramos, Humberto Josué; Moreira, Maurilio Alves; de Barros, Everaldo Gonçalves
- Abstract
The common bean ( Phaseolus vulgaris L.) is the main source of protein and an important source of minerals in several countries around the world. Angular leaf spot, caused by the fungus Pseudocercospora griseola, is one of the major diseases of the common bean. In this work, we used two-dimensional gel electrophoresis and mass spectrometry to analyze alterations in the proteome of common bean leaves challenged with an incompatible race of P. griseola. Twenty-three differentially expressed proteins were detected in leaves of cultivar AND 277 collected at 12, 24 and 48 h after inoculation. The proteins were digested with trypsin and submitted to MALDI-TOF/TOF and MicrOTOF-Q electrospray mass spectrometry. Nineteen of them were identified upon MS/MS fragmentation. Most of these proteins are involved with amino acid metabolism, terpenoid metabolism, phenylpropanoid biosynthesis, antioxidant systems, vitamin and cofactor metabolism, plant–pathogen interaction, carbohydrate metabolism, photosynthesis, or genetic information processing, showing that the interaction in this pathosystem affects different genes from various metabolic pathways and processes.
- Subjects
PLANT proteins; GENE expression in plants; COMMON bean; BACTERIAL blight of cotton; MINERAL content of plants; FUNGAL diseases of plants; ETIOLOGY of diseases; ELECTROPHORESIS; INFORMATION processing
- Publication
Molecular Breeding, 2013, Vol 32, Issue 4, p933
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-013-9922-0