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- Title
Separation of Polyphenols and Caffeine from the Acetone Extract of Fermented Tea Leaves (Camellia sinensis) Using High-Performance Countercurrent Chromatography.
- Authors
Soo Jung Choi; Yong Deog Hong; Bumjin Lee; Jun Seong Park; Hyun Woo Jeong; Wan Gi Kim; Song Seok Shin; Kee Dong Yoon
- Abstract
Leaves from Camellia sienensis are a popular natural source of various beverage worldwide, and contain caffeine and polyphenols derived from catechin analogues. In the current study, caffeine (CAF, 1) and three tea polyphenols including (-)-epigallocatechin 3-O-gallate (EGCg, 2), (-)-gallocatechin 3-O-gallate (GCg, 3), and (-)-epicatechin 3-O-gallate (ECg, 4) were isolated and purified by flow-rate gradient high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:9:1:9, v/v). Two hundred milligrams of acetone-soluble extract from fermented C. sinensis leaves was separated by HPCCC to give 1 (25.4 mg), 2 (16.3 mg), 3 (11.1 mg) and 4 (4.4 mg) with purities over 98%. The structures of 1-4 were elucidated by QTOF-MS, as well as ¹H- and 13C-NMR, and the obtained data were compared to the previously reported values.
- Subjects
TEA; POLYPHENOLS; CAFFEINE; ACETONE; COUNTERCURRENT chromatography
- Publication
Molecules, 2015, Vol 20, Issue 7, p13216
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules200713216