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- Title
Micropropagation of sweet acacia (Vachellia farnesiana), an underutilized ornamental tree.
- Authors
Xu, JianJian; Wilson, Sandra B.; Vendrame, Wagner A.; Beleski, David G.
- Abstract
Sweet acacia (Vachellia farnesiana (L.) Wight and Arn) is an attractive small- to medium-sized evergreen tree native to southern North America spanning from southern California, east to southern Florida. Despite its ornamental appeal, sweet acacia has limited commercial availability. A series of experiments were conducted to determine the effect of hydrothermal scarification on in vitro seed germination, and an efficient level of BA (6-benzylaminopurine) for in vitro multiplication, auxin rooting treatments, and acclimatization of sweet acacia. Results revealed that hydrothermal scarification and visual selection of imbibed seeds are the most effective for rapid in vitro seed germination. The source of seed utilized for the in vitro germination exhibited an unusual characteristic of producing seedlings with 2, 3, or 4 cotyledons. Regardless of the cotyledon number observed, sweet acacia can be efficiently micropropagated using multiplication media supplemented with either 1.0 or 2.0 mg L-1 BA followed by rooting media supplemented 0.5 mg L-1 IBA (indole-3-butyric acid) and 0.5 mg L-1 NAA (1-naphthaleneacetic acid). In vitro derived plantlets were subsequently acclimatized with high survival ranging from 72.7 to 100%.
- Subjects
FLORIDA; ORNAMENTAL trees; COTYLEDONS; ACACIA; SELECTION (Plant breeding); PLANT tissue culture; GERMINATION
- Publication
In Vitro Cellular & Developmental Biology Plant, 2023, Vol 59, Issue 1, p74
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-022-10317-1