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- Title
Site?site communication in the EF-hand Ca<sup>2+</sup>-binding protein calbindin D<sub>9k</sub>.
- Authors
Mäler, Lena; Blankenship, John; Rance, Mark; Chazin, Walter J.
- Abstract
The cooperative binding of Ca2+ ions is an essential functional property of the EF-hand family of Ca2+-binding proteins. To understand how these proteins function, it is essential to characterize intermediate binding states in addition to the apo- and holo-proteins. The three-dimensional solution structure and fast time scale internal motional dynamics of the backbone have been determined for the half-saturated state of the N56A mutant of calbindin D9k with Ca2+ bound only in the N-terminal site. The extent of conformational reorganization and a loss of flexibility in the C-terminal EF-hand upon binding of an ion in the N-terminal EF-hand provide clear evidence of the importance of site?site interactions in this family of proteins, and demonstrates the strength of long-range effects in the cooperative EF-hand Ca2+-binding domain.
- Subjects
BINDING sites; MOLECULAR dynamics; CALCIUM-binding proteins
- Publication
Nature Structural Biology, 2000, Vol 7, Issue 3, p245
- ISSN
1072-8368
- Publication type
Article