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- Title
Effects of targeted temperature management based on different durations after traumatic brain injury in rats.
- Authors
ZHAO Wanyong; SUN Xiaopeng; WANG Jingjing; LI Xiaohong; SUN Hongtao
- Abstract
Objective To investigate the protective effect of target temperature management based different durations with delayed hypothermia for traumatic brain injury(TBI) in rats. Methods 36 healthy adult male SD rats were randomly divided into NT group, HT4h group, HT24h group and HT48h group. The TBI model of rats was prepared with an electronic controllable cortical injury device. NT group was treated with normal temperature (37 ) 4 h after TBI, and the experimental groups were treated with low temperature (33.0 ± 1.0 ) 4 h after TBI for 4 h, 24 h, and 48 h. Three days after TBI, the motor function of the rats in each group was evaluated by beam walking test and inclined-grid climbing test, EB staining was used to measure the blood-brain barrier permeability, the change of hippocampal neurons was observed by Nissl staining, the expression of DCX and GFAP was detected by immunofluorescence, and the expression of Bcl-2 and Bax was measured by Western blot and immunohistochemistry. Results Compared with NT group, the experimental groups could significantly improve the motor function of TBI rats, reduce the permeability of blood-brain barrier,protect hippocampal neurons, promote DCX expression, inhibit GFAP expression, up-regulate the expression of Bcl-2 protein, and down-regulate the expression of Bax protein. However, the protective effect was more apparent in HT48h group than other experimental groups (P < 0.05). Conclusion Long-term delayed target temperature management has a significant brain protective effect.
- Subjects
INDUCED hypothermia; BRAIN injuries; TEMPERATURE effect; LABORATORY rats; RATS; BAX protein
- Publication
Journal of Practical Medicine / Shiyong Yixue Zazhi, 2024, Vol 40, Issue 12, p1625
- ISSN
1006-5725
- Publication type
Article
- DOI
10.3969/j.issn.1006-5725.2024.12.003