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- Title
Production of omega-5 gliadin monoclonal antibodies for allergenic evaluation of WDEIA-causing wheat varieties.
- Authors
Sim, Jae-Ryeong; Lee, Jong-Yeol; Kim, Sewon
- Abstract
In allergic individuals, ingestion of wheat can lead to wheat-dependent exercise-induced anaphylaxis (WDEIA). Many studies have been conducted to find WDEIA allergen–deficient wheat, including by generating omega-5 gliadin antibodies. However, the reported antibodies have not been specific enough to detect omega-5 gliadins encoded on the 1B chromosome. In this study, we generated monoclonal antibodies against the major allergens causing WDEIA, omega-5 gliadins. Using these antibodies (mono-O5B-1C10), we assessed accumulation of omega-5 gliadins in wild-type and nullisomic-tetrasomic (NT) lines of the wheat (Triticum aestivum) varieties Chinese Spring (CS) by one- and two-dimensional gel electrophoresis, followed by Coomassie blue staining or immunoblotting with mono-O5B-1C10. We also tested mono-O5B-1C10 for major omega-5 gliadins in various wheat germplasms. Our results thus demonstrate the specificity of mono-O5B-1C10 for major omega-5 gliadins and potentially useful for identifying of omega-5 gliadin–deficient wheat varieties that should not cause WDEIA.
- Subjects
KUMASI (Ghana); WHEAT breeding; MONOCLONAL antibodies; WHEAT; EXERCISE-induced anaphylaxis; GLIADINS; TWO-dimensional electrophoresis
- Publication
Applied Biological Chemistry, 2023, Vol 66, Issue 1, p1
- ISSN
2468-0834
- Publication type
Article
- DOI
10.1186/s13765-023-00807-0