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- Title
尖孢镰刀菌古巴专化型胱硫醚γ-合成酶的功能分析.
- Authors
刘礼广; 丁兆建; 李梦涵; 吴春花; 谢欣捷; 彭军; 张欣
- Abstract
Methionine plays a crucial role in various biological processes in fungi, bacteria and plants. FgMETB encodes a cystathionine gamma-synthase, which is essential for methionine biosynthesis in Fusarium graminearum. In this study, the deletion mutant strains of FoMETB, a homologue of F. graminearum FgMETB, in Fusarium oxysporum f. sp. cubense race 4 (Foc4) were obtained by using homologous recombination technology. The FoMETB deletion mutant strains were unable to grow on minimal medium containing SO42- as the sole sulphur source. Supplementation of 1 mmol/L methionine, but not 1 mmol/L cysteine, rescued the defect of mycelial growth of FoMETB deletion mutant. These results indicated that the enzyme encoded by FoMETB is involved in conversion of cysteine into methionine. In addition, the FoMETB deletion mutant strains showed significant reduction in aerial hypha growth, conidiation, dry weight of mycelium and virulence attacking banana plantlets. This study indicated that FoMETB plays a critical role in regulation of various biological processes in Foc4. This key synthase FoMETB in methionine synthesis is desirable as a new potential antifungal drug target.
- Subjects
CYSTATHIONINE gamma-lyase; METHIONINE; DRUG target; CYSTEINE; FUSARIUM oxysporum; MYCELIUM; FUSARIUM
- Publication
Mycosystema, 2023, Vol 42, Issue 7, p1575
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.220346