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- Title
A high-resolution melting-based assay for discriminating a native Japanese chicken, the Nagoya breed, using the ABR0417 microsatellite marker.
- Authors
Aoki, Akira; Mori, Yoko; Okamoto, Yoshinori; Jinno, Hideto
- Abstract
The Nagoya breed, so-called Nagoya Cochin, is a native Japanese chicken. As the mislabeling of chickens is a matter of concern, causing economic losses relating to the production of the Nagoya breed, a simple and high-accuracy assay is required to distinguish the Nagoya breed. This study successfully developed a novel screening assay to discriminate the Nagoya breed using a high-resolution melting (HRM) assay. A primer set for HRM analysis was designed to amplify the CA repetition in the ABR0417 microsatellite marker. By comparing the results of the HRM analysis with those of the DNA sequence analysis, we investigated the applicability of the HRM-based assay using four differential chickens of the Nagoya breed and twelve other breeds. In the ABR0417 marker, the sequences of all four Nagoya breed chickens were identical to the ABR0417 reference sequence of the Nagoya breed, whereas the sequences for the twelve other chickens were different from those of the Nagoya breed chickens. The HRM analysis of the 16 chickens revealed that the melting curve and peak plots of the Nagoya breed chickens were distinct from those of other chickens. These results demonstrate that this HRM-based method is a simple genetic test to identify the Nagoya breed using the ABR0417 marker.
- Subjects
NAGOYA-shi (Japan); CHICKENS; MICROSATELLITE repeats; CHICKEN breeds; DNA sequencing; POULTRY breeding; GENETIC testing
- Publication
European Food Research & Technology, 2024, Vol 250, Issue 3, p745
- ISSN
1438-2377
- Publication type
Article
- DOI
10.1007/s00217-023-04433-4