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- Title
Importance of conformation for the IgE reactivity of sarcoplasmic calcium-binding protein from the black tiger shrimp Penaeus monodon.
- Authors
Morii, Aika; Mita, Hajime; Ishizaki, Shoichiro; Shiomi, Kazuo
- Abstract
Sarcoplasmic calcium-binding protein (SCP) is an EF-hand Ca-binding protein recently identified as a new crustacean allergen. Some EF-hand Ca-binding allergens, such as parvalbumin (fish allergen) and Bet v 4 (birch pollen allergen), have been shown to contain conformational-type IgE epitopes associated with the Ca-chelating. This study was performed to clarify the relationships between IgE reactivity and structure of the black tiger shrimp SCP. When analyzed by ELISA in the absence and presence of EGTA, the IgE reactivity of the black tiger shrimp SCP was found to be considerably reduced by Ca-depletion. Furthermore, circular dichroism spectral data showed a conformational difference between Ca-bound and Ca-depleted forms of the black tiger shrimp SCP. On the other hand, the synthetic 18 peptides spanning the entire amino acid sequence of the black tiger shrimp SCP were all assessed to have little IgE-binding ability by ELISA. Our results demonstrate that the IgE reactivity of the black tiger shrimp SCP is attributable mostly to conformational-type IgE epitopes, at least part of which is maintained by Ca-chelating.
- Subjects
IMMUNOGLOBULIN E; SARCOPLASMIC reticulum; CALCIUM-binding proteins; PROTEIN conformation; ALLERGENS; PENAEUS monodon; PARVALBUMINS; CIRCULAR dichroism
- Publication
European Food Research & Technology, 2013, Vol 236, Issue 1, p165
- ISSN
1438-2377
- Publication type
Article
- DOI
10.1007/s00217-012-1867-8