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- Title
Lipid biosynthesis from [<sup>14</sup> C]-glucose in the coral Montipora digitata.
- Authors
OKU, Hirosuke; YAMASHIRO, Hideyuki; ONAGA, Kyoko
- Abstract
Glucose has been implicated in functioning as a form of carbon translocated from symbiont zooxanthellae to the host coral cell. The present paper describes the lipid biosynthesis from [14 C]-glucose in the coral tissue. To study the incorporation of [14 C]-glucose into lipids, the branch tips of the coral Montipora digitata were incubated with [14 C]-glucose or another radiolabeled substrates. The lipid biosynthesis from [14 C]-glucose was dependent on light, and was decreased by dark conditions or by photosystem II inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Of the lipid classes, the light dependency was more pronounced with the biosynthesis of triacylglycerols (TG) and wax compared with phospholipids. Examination of [14 C]-label distribution in the glycerolipids suggested that [14 C]-glucose supplied mainly the fatty acid moiety of newly synthesized TG, while [14 C]-glucose provided evenly the fatty acid moiety and the glycerol skeleton of phospholipids. The comparison of [14 C]-labeling of lipid from host coral tissue and symbiont zooxanthellae suggested that [14 C]-glucose entered the coral cell and was processed in parallel in the zooxanthellae and host cells. Furthermore, the coral cells used various [14 C]-labeled sugars for lipid synthesis with similar lipid labeling profile as was the case for glucose. The current study thus supports the view that the low-molecular-weight compound, sugars and amino acids, once translocated from zooxanthellae to host cell were metabolized toward lipogenesis as well as glycerol.
- Subjects
GLUCOSE; CORALS; PHOSPHOLIPIDS; FATTY acids; ZOOXANTHELLA
- Publication
Fisheries Science, 2003, Vol 69, Issue 3, p625
- ISSN
0919-9268
- Publication type
Article
- DOI
10.1046/j.1444-2906.2003.00665.x