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- Title
The WUSCHEL- RELATED HOMEOBOX 3 gene Pa WOX3 regulates lateral organ formation in Norway spruce.
- Authors
Alvarez, José M.; Sohlberg, Joel; Engström, Peter; Zhu, Tianqing; Englund, Marie; Moschou, Panagiotis N.; Arnold, Sara
- Abstract
In angiosperms, WUSCHEL- RELATED HOMEOBOX 3 ( WOX3) genes are required for the recruitment of founder cells from the lateral domains of shoot meristems that form lateral regions of leaves. However, the regulation of the formation of lateral organs in gymnosperms remains unknown., By using somatic embryos of Norway spruce ( Picea abies) we have studied the expression and function of Pa WOX3 during embryo development. The mRNA abundance of Pa WOX3 was determined by quantitative real-time PCR, and the spatial expression of Pa WOX3 was analysed by histochemical β -glucuronidase ( GUS) assays and in situ mRNA hybridization. To investigate the function of Pa WOX3, we analysed how downregulation of Pa WOX3 in RNA interference lines affected embryo development and morphology., Pa WOX3 was highly expressed in mature embryos at the base of each cotyledon close to the junction between the cotyledons, and in the lateral margins of cotyledons and needles, separating them into an adaxial and an abaxial side. Downregulation of the expression of Pa WOX3 caused defects in lateral margin outgrowth in cotyledons and needles, and reduced root elongation., Our data suggest that the WOX3 function in margin outgrowth in lateral organs is conserved among the seed plants, whereas its function in root elongation may be unique to gymnosperms.
- Subjects
HOMEOBOX genes of plants; NORWAY spruce; MERISTEMS; MESSENGER RNA; GENE expression in plants; COTYLEDONS
- Publication
New Phytologist, 2015, Vol 208, Issue 4, p1078
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.13536