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- Title
Two amino acid substitutions in acetylcholinesterase associated with pirimicarb and organophosphorous insecticide resistance in the cotton aphid,Aphis gossypiiGlover (Homoptera: Aphididae).
- Authors
Toda, S.; Komazaki, S.; Tomita, T.; Kono, Y.
- Abstract
The complete coding sequences of two acetylcholinesterase (AChE) genes,Ace1(orthologous toDrosophila Ace) andAce2(paralogous toAce), from the cotton aphid (Aphis gossypii) were identified and sequences from carbamate resistant and susceptible strains compared. No change in the amino acid sequences was found inAce1, while two amino acid substitutions, Ser431Phe and Ala302Ser, were detected between resistant and susceptible strains inAce2. The position of Ser431Phe corresponds to one of fourteen aromatic residues lining the active site gorge and is located in the acyl pocket. Ala302Ser is located at one of the three residues which form the oxyanion hole in the active site of AChE. The Ser431Phe and Ala302Ser substitutions may play a role in pirimicarb and organophosphate resistance, respectively.
- Subjects
AMINO acids; ACETYLCHOLINESTERASE; INSECTICIDE resistance; COTTON aphid; CARBAMATES; PHOSPHATES
- Publication
Insect Molecular Biology, 2004, Vol 13, Issue 5, p549
- ISSN
0962-1075
- Publication type
Article
- DOI
10.1111/j.0962-1075.2004.00513.x