We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Lateral interactions between CD276 and CD147 are essential for stemness in breast cancer: a novel insight from proximal proteome analysis.
- Authors
Seo, Yu Ri; Lee, Junghyeon; Ryu, Han Suk; Kim, EunHee G.; Kim, So Hyun; Jeong, Jieun; Jung, Hyeryeon; Jung, YeoJin; Kim, Han Byeol; Jo, Yeon Hui; Kim, Yeong Dong; Jin, Min-Sun; Lee, Yong Yook; Kim, Kristine M.; Yi, Eugene C.
- Abstract
Oncogenic cell-surface membrane proteins contribute to the phenotypic and functional characteristics of cancer stem cells (CSCs). We employed a proximity-labeling proteomic approach to quantitatively analyze the cell-surface membrane proteins in close proximity to CD147 in CSCs. Furthermore, we compared CSCs to non-CSCs to identify CSC-specific cell-surface membrane proteins that are closely interact with CD147 and revealed that lateral interaction between CD147 and CD276 concealed within the lipid raft microdomain in CSCs, confers resistance to docetaxel, a commonly used chemotherapy agent for various cancer types, including metastatic breast cancer. Moreover, we investigated the clinical relevance of CD147 and CD276 co-expression in HER2+ breast cancer (BC) and triple-negative breast cancer patients who underwent chemotherapy. We observed poor disease-free survival and Overall survival rates in patients of CD147 and CD276 (p = 0.04 and 0.08, respectively). Subsequent immunohistochemical analysis in independent cohorts of HER2+ BC support for the association between co-expression of CD147 and CD276 and a poor response to chemotherapy. Collectively, our study suggests that the lateral interaction between CD147 and its proximal partners, such as CD276, may serve as a poor prognostic factor in BC and a predictive marker for the critical phenotypic determinant of BC stemness.
- Subjects
HER2 positive breast cancer; BREAST cancer; TRIPLE-negative breast cancer; METASTATIC breast cancer; LIPID rafts; PROTEOMICS; BREAST
- Publication
Scientific Reports, 2023, Vol 13, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-023-41416-7