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- Title
Anti-Diabetic Atherosclerosis Effect of Prunella vulgaris in db/db Mice with Type 2 Diabetes.
- Authors
Hwang, Sun Mi; Kim, Jin Sook; Lee, Yun Jung; Yoon, Jung Joo; Lee, So Min; Kang, Dae Gill; Lee, Ho Sub
- Abstract
Diabetes mellitus is the leading cause of vascular complications such as atherosclerosis. This study was designed to investigate whether Prunella vulgaris (APV) would inhibit diabetic atherosclerosis in db/db mice with type 2 diabetes. The db/db mice were treated with high fat/high cholesterol (HFHC) diet and an aqueous extract of APV (100 and 200 mg/kg/day) for eight weeks to examine the long-term effect on metabolic abnormalities and diabetic atherosclerosis. APV treatment markedly lowered blood glucose and systolic blood pressure. The db/db mice experienced an increase in blood urea nitrogen as well as a decrease of creatinine clearance, the latter of which was restored by treatment with APV. Treatment with APV markedly decreased total plasma cholesterol, triglyceride, and LDL-cholesterol and also increased the HDL-cholesterol. In addition, malondialdehyde and TGF-β1 were decreased by treatment of APV. On the other hand, total NO level was decreased in db/db mice. However, the NO level was increased by treatment with APV, suggesting an association with vascular dysfunction. Vascular relaxation of aortic rings by acetylcholine or SNP-inducement was ameliorated by APV in a dose-dependent manner. Damage of vascular intima and hypertrophic of media were observed in db/db mice; however its dysfunction was improved by the treatment of APV. APV treatment significantly reduced the aortic expressions of ICAM-1, VCAM-1, ET-1, and nitrotyrosine. Furthermore, expression of eNOS in aortic was remarkably increased by APV treatment. Taken together, APV suppressed hyperglycemia and diabetic vascular dysfunction in HFHC diet-db/db mice. The present data suggest that Prunella vulgaris may prevent development of diabetic atherosclerosis.
- Subjects
MEDICAL botany; HYPOGLYCEMIC agents; THERAPEUTIC use of plant extracts; ANALYSIS of variance; ANIMAL experimentation; ATHEROSCLEROSIS; BLOOD testing; CARDIOVASCULAR system; CELL culture; ENZYME-linked immunosorbent assay; IMMUNOHISTOCHEMISTRY; ASIAN medicine; METABOLISM; MICE; TYPE 2 diabetes; RESEARCH funding; STAINS &; staining (Microscopy); T-test (Statistics); DESCRIPTIVE statistics
- Publication
American Journal of Chinese Medicine, 2012, Vol 40, Issue 5, p937
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X12500693