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- Title
Vasorelaxant Effect of Cinnamomi Ramulus Ethanol Extract via Rho-Kinase Signaling Pathway.
- Authors
Kang, Yun Hwan; Shin, Heung Mook
- Abstract
The Rho-kinase (ROCK) signaling pathway is substantially involved in vascular contraction. This study investigated the vasodilatory effects and possible mechanisms of Cinnamomi ramulus ethanol extract (CRE), with the hypothesis that the CRE vasodilatory effect involves RhoA and the ROCK signaling pathway in rat aortic preparations. CRE (0.05-1 mg/ml) dose-dependently relaxed the vascular contraction induced by phenylephrine and calpeptin in an endothelium-independent manner. Measurement of the expression levels of ROCK-related signaling molecules in response to calpeptin revealed that CRE completely inhibited RhoA and ROCK2 protein expressions. Furthermore, CRE dephosphorylated the subsequent downstream targets myosin phosphatase targeting subunit 1 (MYPT-1), protein kinase C potentiated phosphatase inhibitor protein-17 kDa (CPI-17) and myosin light chain 20 kDa (MLC20). We conclude that the vasorelaxation effect of CRE occurs via downregulation of ROCK signal molecules.
- Subjects
THERAPEUTIC use of plant extracts; ANALYSIS of variance; ANIMAL experimentation; AORTA; BIOLOGICAL assay; VASODILATION; CELL surface antigens; CINNAMON; DOSE-effect relationship in pharmacology; ELECTROPHYSIOLOGY; ETHANOL; GAS chromatography; HYPERTENSION; IMMUNODIAGNOSIS; MASS spectrometry; METABOLISM; PHOSPHOTRANSFERASES; RATS; RESEARCH funding; T-test (Statistics); WESTERN immunoblotting; THERAPEUTICS
- Publication
American Journal of Chinese Medicine, 2011, Vol 39, Issue 5, p867
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X11009263