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- Title
Quantitative assessment of micronuclei in oral exfoliative buccal squamous cells in smokers, chewers and healthy individuals using Giemsa Stain and Papainicolous Stain.
- Authors
Joshi, Bharath; Kattappagari, Kiran Kumar; Lingamaneni, Krishna Prasad; Baddam, Venkat Ramana Reddy
- Abstract
Brief Background The main reason for oral cancer is tobacco consumption in various forms, such as chewing and smoking. Micronuclei in oral exfoliated cells are markers of chromosomal damage caused by toxic genetic agents from tobacco-related substances, alcohol, etc. The number of micronuclei indicates the cell's genotoxic effect and can be used as a biomarker to assess the changes in the cellular changes. Recently research is focused on determining early genotoxicity changes using exfoliative cytology in human buccal epithelial cells. Aim Quantitative analysis of micronuclei in oral exfoliated oral, buccal mucosal cells using Papainicolous stain with Giemsa stain in tobacco users in the form of chewing and smoking in comparison with healthy individuals. Materials and Methods A total of seventy-five cases were selected for this study. The seventy-five participants were divided into three groups based on the habits; tobacco smokers (n=25), tobacco chewers (n=25), and healthy individuals (n=25). Results The mean number of micronuclei in Giemsa stain was 255.2 ± 78 compared with PAP stain 155.12 ± 78.5. The increased mean number of micronuclei in chewers 273.24 ± 80.7, and different stain comparison Giemsa stain showed highest in chewers. A mean number of micronuclei showed statistically highly significant (p=0.05). Pair-wise comparison of micronuclei inter and intra groups in all three groups, smokers and chewers showed highly statistically substantial (p= 0.0001) compared with healthy individuals. Summary and Conclusion Micronuclei provide evidence that consumption of smokeless tobacco may be developing oral cancer, and micronuclei assay can be used as biomarkers of genotoxicity and carcinogenesis progression.
- Subjects
STAINS &; staining (Microscopy); NUCLEOLUS; SMOKELESS tobacco; TOBACCO use; EXFOLIATIVE cytology
- Publication
Clinical Dentistry (0974-3979), 2021, Vol 15, Issue 9, p12
- ISSN
0974-3979
- Publication type
Article
- DOI
10.33882/ClinicalDent.15.30003