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- Title
Changes in Neuronal Signaling and Cell Stress Response Pathways are Associated with aMultigenic Response of Drosophila melanogaster to DDT Selection.
- Authors
Seong, Keon Mook; Coates, Brad S.; Sun, Weilin; Clark, John M.; Pittendrigh, Barry R.
- Abstract
The adaptation of insect populations to insecticidal control is a continual threat to human health and sustainable agricultural practices, butmany complex genomicmechanisms involved in this adaption remain poorly understood. This study applied a systems approach to investigate the interconnections between structural and functional variance in response to dichlorodiphenyltrichloroethane (DDT) within the Drosophila melanogaster strain 91-R. Directional selection in 6 selective sweeps coincided with constitutive gene expression differences in DDT resistant flies, including the most highly upregulated transcript, Unc-115 b, which plays a central role in axon guidance, and the most highly downregulated transcript, the angiopoietin-like CG31832, which is involved in directing vascular branching and dendrite outgrowth but likely may be under trans-regulatory control. Direct functions and protein--protein interactions mediated by differentially expressed transcripts control changes in cellmigration, signal transduction, and gene regulatory cascades that impact the nervous system. Although changes to cellular stress response pathways involve 8 different cytochrome P450s, stress response, and apoptosis is controlled by a multifacetted regulatory mechanism. These data demonstrate that DDT selection in 91-R may have resulted in genome-wide adaptations that impacts genetic and signal transduction pathways that converge to modify stress response, cell survival, and neurological functions. This study implicates the involvement of a multigenic mechanism in the adaptation to a chemical insecticide, which impact interconnected regulatory cascades. We propose that DDT selection within 91-R might act systemically, wherein pathway interactions function to reinforce the epistatic effects of individual adaptive changes on an additive or nonadditive basis.
- Subjects
INSECT adaptation; DDT (Insecticide); DROSOPHILA; GENE expression; ANGIOPOIETINS; CELL migration
- Publication
Genome Biology & Evolution, 2017, Vol 9, Issue 12, p3356
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evx252