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- Title
Osteogenic Induction of Bone Marrow-Derived Stromal Cells on Simvastatin-Releasing, Biodegradable, Nano- to Microscale Fiber Scaffolds.
- Authors
Wadagaki, Ryu; Mizuno, Daiki; Yamawaki-Ogata, Aika; Satake, Makoto; Kaneko, Hiroaki; Hagiwara, Sumitaka; Yamamoto, Noriyuki; Narita, Yuji; Hibi, Hideharu; Ueda, Minoru
- Abstract
Tissue engineering is an effective approach for the treatment of bone defects. Statins have been demonstrated to promote osteoblastic differentiation of bone marrow-derived stromal cells (BMSCs). Electrospun biodegradable fibers have also shown applicability to drug delivery in the form of bone tissue engineered scaffolds with nano- to microscale topography and high porosity similar to the natural extracellular matrix (ECM). The aim of this study was to investigate the feasibility of a simvastatin-releasing, biodegradable, nano- to microscale fiber scaffold (SRBFS) for bone tissue engineering with BMSCs. Simvastatin was released from SRBFS slowly. BMSCs were observed to spread actively and rigidly adhere to SRBFS. BMSCs on SRBFS showed an increase in alkaline phosphatase activity 2 weeks after cell culture. Furthermore, osteoclastogenesis was suppressed by SRBFS in vitro. The new bone formation and mineralization in the SRBFS group were significantly better than in the biodegradable fiber scaffold (BFS) without simvastatin 12 weeks after implantation of the cell-scaffold construct into an ectopic site on the murine back. These results suggest that SRBFS promoted osteoblastic differentiation of BMSCs in vitro and in vivo, and demonstrate feasibility as a bone engineering scaffold.
- Subjects
MESENCHYMAL stem cells; ANTILIPEMIC agents; BONE abnormalities; TISSUE engineering; CELL differentiation; BIODEGRADATION; ENZYME activation; FEASIBILITY studies; THERAPEUTICS
- Publication
Annals of Biomedical Engineering, 2011, Vol 39, Issue 7, p1872
- ISSN
0090-6964
- Publication type
Article
- DOI
10.1007/s10439-011-0327-0