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- Title
The RING Finger E3 Ligase SpRing is a Positive Regulator of Salt Stress Signaling in Salt-Tolerant Wild Tomato Species.
- Authors
Shilian Qi; Qingfang Lin; Huishan Zhu; Fenghua Gao; Wenhao Zhang; Xuejun Hua
- Abstract
Protein ubiquitination in plants plays critical roles in many biological processes, including adaptation to abiotic stresses. Previously, RING finger E3 ligase has been characterized during salt stress response in several plant species, but little is known about its function in tomato. Here, we report that SpRing, a stress-inducible gene, is involved in salt stress signaling in wild tomato species Solanum pimpinellifolium 'PI365967'. In vitro ubiquitination assay revealed that SpRing is an E3 ubiquitin ligase and the RING finger conserved region is required for its activity. SpRing is expressed in all tissues of wild tomato and up-regulated by salt, drought and osmotic stresses, but repressed by low temperature. Green fluorescent protein (GFP) fusion analysis showed that SpRing is localized at the endoplasmic reticulum. Silencing of SpRing through a virus-induced gene silencing approach led to increased sensitivity to salt stress in wild tomato. Overexpression of SpRing in Arabidopsis thaliana resulted in enhanced salt tolerance during seed germination and early seedling development. The expression levels of certain key stress-related genes are altered both in SpRing-overexpressing Arabidopsis plants and virus-induced gene silenced tomato seedlings. Taken together, our results indicate that SpRing is involved in salt stress and functions as a positive regulator of salt tolerance.
- Subjects
LIGASES; HALOPHYTES; TOMATOES; UBIQUITINATION; PLANT adaptation; ABIOTIC stress; UBIQUITIN ligases; GREEN fluorescent protein
- Publication
Plant & Cell Physiology, 2016, Vol 57, Issue 3, p528
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pcw006