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- Title
A Critical Role for IL-21 Receptor Signaling in the Coxsackievirus B3-Induced Myocarditis.
- Authors
Yang, Fan; Wei, Xiao-mou; Liang, Wen-wu; Mo, Wen-hong; Tan, Bao-ping; Wang, Hong
- Abstract
To determine whether IL-21 receptor signaling plays a significant role in promoting Tfh cell-mediated cardiac injury in viral myocarditis (VMC), we compared IL-21R-deficient mice for some parameters of VMC. Balb/c and IL-21R mice were infected with CVB3. Frequencies of splenic Tfh cells were determined by flow cytometric analysis, and productions of anti-adenine nucleotide translocator (ANT) autoantibodies were detected by enzyme-linked immunosorbent assay. To determine the effects of IL-21R signal on the proliferation of B cells, lymphocytes from spleens of the IL-21R and Balb/c mice infected by CVB3 were tagged with carboxyfluorescein succinimidyl ester (CFSE) and then were stimulated with lipopolysaccharides plus IL-21 or anti-IL-21 neutralizing antibody for 3 days. The proliferation of B cells was analyzed by flow cytometry. Anti-ANT antibodies in the supernatants were detected by ELISA. Results showed that IL-21R mice developed significantly less inflammation of the myocardium than Balb/c mice. Numbers of the Tfh cells and levels of anti-ANT antibody were decreased in IL-21R mice, indicating IL-21 signaling plays a role on the Tfh cell response. The percentage of CD19CFSE B cells decreased in IL-21R mice compared to VMC mice. And anti-ANT antibodies were detected at lower levels in cultured supernatant from IL-21R mice than in those from VMC mice. These data suggest that IL-21R signal may contribute to anti-ANT antibody production and expansion of B cells in VMC mice.
- Subjects
COXSACKIEVIRUS diseases; MYOCARDITIS; AUTOANTIBODIES; ENZYME-linked immunosorbent assay; LIPOPOLYSACCHARIDES; THERAPEUTICS
- Publication
Inflammation, 2017, Vol 40, Issue 4, p1428
- ISSN
0360-3997
- Publication type
Article
- DOI
10.1007/s10753-017-0586-5