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- Title
Cytotoxicity of cleaning agents for ocular prostheses.
- Authors
Coelho Goiato, Marcelo; Andreotti, Agda Marobo; Pereira de Caxias, Fernanda; Freitas da Silva, Emily Vivianne; de Oliveira Gonçalves, Letícia; Penha de Oliveira, Sandra Helena; Balera Brito, Victor Gustavo; de Moraes Melo Neto, Clóvis Lamartine; Micheline dos Santos, Daniela
- Abstract
Background. Polymethylmethacrylate (PMMA) is the most used material for the manufacturing of eye prostheses. Objectives. To investigate the cytotoxicity of different cleaning agents for ocular prostheses on human conjunctival cells. Materials and methods. Six groups ofspecimens were created (saline, soap, 4% chlorhexidine, hydrogen peroxide, 1% triclosan, and citronella oil). Three specimens were made for each disinfectant ateach disinfection period (1, 7, 15, 30, 60, and 90 days), totaling 108 specimens. Thus, the specimens were disinfected, with different disinfectants, for different periods of time. After each disinfection process, thespecimens were washed with sterile distilled water. A human conjunctival cell line was grown ontheacrylic resin specimens and then cytotoxicity tests (MTT and Neutral Red (NR)) were performed. A negative control (untreated cell cultures) and positive control (Tween 20) were created. Two-way analysis ofvariance (ANOVA) and Bonferroni test were performed (p < 0.05). Results. For the MTT and NR tests, when there was a significant difference between thedisinfectant and negative control, thedisinfectant generated asignificant reduction incell proliferation most of thetime. Conclusions. All reductions in cell proliferation caused by the disinfectants were clinically acceptable. Alldisinfectants tested inthis study were found tobe non-cytotoxic tohuman conjunctival cells.
- Subjects
CLEANING compounds; PROSTHETICS; DISINFECTION &; disinfectants; HYDROGEN peroxide; POLYMETHYLMETHACRYLATE; MOUTHWASHES
- Publication
Polimery w Medycynie, 2023, Vol 53, Issue 1, p37
- ISSN
0370-0747
- Publication type
Article
- DOI
10.17219/pim/163118