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- Title
Morpho-Cytometric Investigations on Haemolymph Collected from Honeybees Originated from South of Romania.
- Authors
Šapcaliu, Agripina; Pavel, Crenguţa; Căwa, Eliza; Rădoi, Ion; Tudor, Nicolae; Tudor, Poliana; Milea, Florin
- Abstract
The studies were carried out in 2007-2009, in the frame of the Pathology Department belonging to the Beekeeping Research and Development Institute-Bucharest. There were performed complex morpho-cytological investigations on haemolymph samples collected from honeybees originated from honeybee colonies located in the southern part of Romania. These researches mainly aimed to identify and classify the haemocytes according to the literature, to study the morphological characters of haemocytes, to establish an optimal staining method for morphologic studies, to study the stage transformation of haemocytes, to highlight the phagocytosis process in natural bacterial infection. The haemolymph samples were collected from healthy and natural infected honeybees and slides were prepared using the following staining methods: Giemsa, May Grunwald- Giemsa and May Grunwald-Giemsa modified and adapted for the haemocytes sensitivity testing. The following parameters of the haemocytes from centrifuged and non-centrifuged haemolymph were investigated and described: the density and their type, percentage of the phagocytes and non-phagocytes in honeybees from healthy and natural infected colonies, stages of haemocytes formation by morphometrical analyses using a specific measurement software.
- Subjects
ROMANIA; BEEKEEPING; HONEYBEES; MORPHIDAE; ANTIGEN-antibody reactions; IMMUNE system
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Animal Science & Biotechnologies, 2009, Vol 66, Issue 1/2, p270
- ISSN
1843-5262
- Publication type
Article