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- Title
Increased image depth for epi-illumination microscopy.
- Authors
Wilson, Trevor C.; Charlton, W. A.; Posluszny, Usher
- Abstract
Epi-illumination microscopy is a useful tool for studying morphology, but its use is limited by a small depth of field. Using digital editing, we have enhanced the depth of field for epi-illumination micrographs obtained for a number of shoot apices. For each specimen, an epi-illumination microscope fitted with Leitz Ultropak dipping cones was used to capture a series of digital images at successive levels of focus. Adobe® Photoshop® was used to produce one image by assembling only the focused areas of the image series. This stacking method offers a better way to present epi-illumination micrographs and increases the microscope’s usefulness as an alternative for conventional scanning electron microscopy. The method could be applied to images collected by other microscopical techniques.
- Subjects
PLANT morphology; MICROSCOPY; MORPHOLOGY; SCANNING electron microscopy; DIGITAL photography; SHOOT apexes; PHOTOGRAPHY; SHOOT apical meristems; DIGITAL images
- Publication
Canadian Journal of Botany, 2006, Vol 84, Issue 3, p508
- ISSN
0008-4026
- Publication type
Article
- DOI
10.1139/B06-018