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- Title
Interactions of [ NSI] prion-like determinant with SUP35 and VTS1 genes in Saccharomyces cerevisiae.
- Authors
Nizhnikov, A.; Kondrashkina, A.; Galkin, A.
- Abstract
Previously we characterized [ NSI], determinant, that possesses the features of a yeast prion. This determinant causes the nonsense suppression in strains that bear different N-substituted variants of Sup35p, which is a translation release factor eRF3. As a result of the genomic screen, we identified VTS1, the overexpression of which is a phenotypic copy of [ NSI]. Here, we analyzed the influence of SUP35 and VTS1 on [ NSI]. We demonstrated nonsense suppression in the [ NSI] strains, which appears when SUP35 expression was decreased or against a background of general defects in the fidelity of translation termination. [ NSI] has also been shown to increase VTS1 mRNA amounts. These findings facilitate the insight into the mechanisms of nonsense suppression in the [ NSI] strains and narrow the range of candidates for [ NSI] determinant.
- Subjects
SACCHAROMYCES cerevisiae; PHENOTYPES; GENE expression; MESSENGER RNA; GENOMICS; NONSENSE suppression (Genetics)
- Publication
Russian Journal of Genetics, 2013, Vol 49, Issue 10, p1004
- ISSN
1022-7954
- Publication type
Article
- DOI
10.1134/S1022795413100074