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- Title
Domain Localization by Graphene Oxide in Supported Lipid Bilayers.
- Authors
Tero, Ryugo; Hagiwara, Yoshi; Saito, Shun
- Abstract
The gel-phase domains in a binary supported lipid bilayer (SLB) comprising dioleoylphosphatidylcholine (DOPC) and dipalmitoylphosphatidylcholine (DPPC) were localized on graphene oxide (GO) deposited on a SiO2/Si substrate. We investigated the distribution of the gel-phase domains and the liquid crystalline (Lα) phase regions in DOPC+DPPC-SLB on thermally oxidized SiO2/Si substrates with GO flakes to understand the mechanism of the domain localization on GO. Fluorescence microscopy and atomic force microscopy revealed that the gel-phase domains preferably distributed on GO flakes, whereas the fraction of the Lα-phase increased on the bare SiO2 surface which was not covered with the GO flakes. The gel-phase domain was condensed on GO more effectively at the lower cooling rate. We propose that nucleation of the gel-phase domain preferentially occurred on GO, whose surface has amphiphilic property, during the gel-phase domain formation. The domains of the liquid ordered (Lo) phase were also condensed on GO in a ternary bilayer containing cholesterol that was phase-separated to the Lo phase and the liquid disordered phase. Rigid domains segregates on GO during their formation process, leaving fluid components to the surrounding region of GO.
- Subjects
GRAPHENE oxide; BILAYER lipid membranes; ATOMIC force microscopy; FLUORESCENCE microscopy; LIPID rafts; CHOLESTEROL
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 9, p7999
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24097999