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- Title
Isolation and Molecular Characterization of 1-Aminocyclopropane-1-carboxylic Acid Synthase Genes in Hevea brasiliensis.
- Authors
Jia-Hong Zhu; Jing Xu; Wen-Jun Chang; Zhi-Li Zhang
- Abstract
Ethylene is an important factor that stimulates Hevea brasiliensis to produce natural rubber. 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is a rate-limiting enzyme in ethylene biosynthesis. However, knowledge of the ACS gene family of H. brasiliensis is limited. In this study, nine ACS-like genes were identified in H. brasiliensis. Sequence and phylogenetic analysis results confirmed that seven isozymes (HbACS1-7) of these nine ACS-like genes were similar to ACS isozymes with ACS activity in other plants. Expression analysis results showed that seven ACS genes were differentially expressed in roots, barks, flowers, and leaves of H. brasiliensis. However, no or low ACS gene expression was detected in the latex of H. brasiliensis. Moreover, seven genes were differentially up-regulated by ethylene treatment. These results provided relevant information to help determine the functions of the ACS gene in H. brasiliensis, particularly the functions in regulating ethylene stimulation of latex production.
- Subjects
CHEMICAL synthesis; CARBOXYLIC acids; HEVEA; PLANT genetics; PLANT molecular biology; GENE expression in plants; BOTANICAL chemistry
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 2, p4136
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms16024136