We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The overexpression of peroxiredoxin-4 affects the progression of idiopathic pulmonary fibrosis.
- Authors
Hanaka, Tetsuya; Kido, Takashi; Noguchi, Shingo; Yamada, Sohsuke; Noguchi, Hirotsugu; Guo, Xin; Nawata, Aya; Wang, Ke-Yong; Oda, Keishi; Takaki, Tsutomu; Izumi, Hiroto; Ishimoto, Hiroshi; Yatera, Kazuhiro; Mukae, Hiroshi
- Abstract
<bold>Background: </bold>Acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) is life-threatening. Several serum biomarkers, such as Krebs von den Lungen-6 (KL-6) and surfactant protein D (SP-D), are clinically used for evaluating AE-IPF, but these biomarkers are not adequate for establishing an early and accurate diagnosis of AE-IPF. Recently, the protective roles of the members of the peroxiredoxin (PRDX) family have been reported in IPF; however, the role of PRDX4 in AE-IPF is unclear.<bold>Methods: </bold>Serum levels of PRDX4 protein, KL-6, SP-D and lactate dehydrogenase (LDH) in 51 patients with stable IPF (S-IPF), 38 patients with AE-IPF and 15 healthy volunteers were retrospectively assessed using enzyme-linked immunosorbent assay. Moreover, as an animal model of pulmonary fibrosis, wild-type (WT) and PRDX4-transgenic (Tg) mice were intratracheally administered with bleomycin (BLM, 2 mg/kg), and fibrotic and inflammatory changes in lungs were evaluated 3 weeks after the intratracheal administration.<bold>Results: </bold>Serum levels of PRDX4 protein, KL-6, SP-D and LDH in patients with S-IPF and AE-IPF were significantly higher than those in healthy volunteers, and those in AE-IPF patients were the highest among the three groups. Using receiver operating characteristic curves, area under the curve values of serum PRDX4 protein, KL-6, SP-D, and LDH for detecting AE-IPF were 0.873, 0.698, 0.675, and 0.906, respectively. BLM-treated Tg mice demonstrated aggravated histopathological findings and poor prognosis compared with BLM-treated WT mice. Moreover, PRDX4 expression was observed in alveolar macrophages and lung epithelial cells of BLM-treated Tg mice.<bold>Conclusions: </bold>PRDX4 is associated with the aggravation of inflammatory changes and fibrosis in the pathogenesis of IPF, and serum PRDX4 may be useful in clinical practice of IPF patients.
- Subjects
UNITED States. Bureau of Land Management; PULMONARY fibrosis; IDIOPATHIC pulmonary fibrosis; RECEIVER operating characteristic curves; BLOOD proteins; PULMONARY surfactant-associated protein D; ENZYME-linked immunosorbent assay
- Publication
BMC Pulmonary Medicine, 2019, Vol 19, Issue 1, p1
- ISSN
1471-2466
- Publication type
journal article
- DOI
10.1186/s12890-019-1032-2