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- Title
An EST resource for tilapia based on 17 normalized libraries and assembly of 116,899 sequence tags.
- Authors
Bo-Young Lee; Howe, Aimee E.; Conte, Matthew A.; D'Cotta, Helena; Pepey, Elodie; Baroiller, Jean-Francois; di Palma, Federica; Carleton, Karen L.; Kocher, Thomas D.
- Abstract
Background: Large collections of expressed sequence tags (ESTs) are a fundamental resource for analysis of gene expression and annotation of genome sequences. We generated 116,899 ESTs from 17 normalized and two nonnormalized cDNA libraries representing 16 tissues from tilapia, a cichlid fish widely used in aquaculture and biological research. Results: The ESTs were assembled into 20,190 contigs and 36,028 singletons for a total of 56,218 unique sequences and a total assembled length of 35,168,415 bp. Over the whole project, a unique sequence was discovered for every 2.079 sequence reads. 17,722 (31.5%) of these unique sequences had significant BLAST hits (e-value < 10-10) to the UniProt database. Conclusion: Normalization of the cDNA pools with double-stranded nuclease allowed us to efficiently sequence a large collection of ESTs. These sequences are an important resource for studies of gene expression, comparative mapping and annotation of the forthcoming tilapia genome sequence.
- Subjects
TILAPIA; COMPLEMENTARY DNA; GENE libraries; SEQUENCE alignment; GENE expression; GENOMES
- Publication
BMC Genomics, 2010, Vol 11, p278
- ISSN
1471-2164
- Publication type
Article
- DOI
10.1186/1471-2164-11-278