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- Title
Hemoglobin regulates the migration of glioma cells along poly(ε-caprolactone)-aligned nanofibers.
- Authors
Roth, Alexander D.; Elmer, Jacob; Harris, David R.; Huntley, Joseph; Palmer, Andre F.; Nelson, Tyler; Johnson, Jed K.; Xue, Ruipeng; Lannutti, John J.; Viapiano, Mariano S.
- Abstract
Aligned fibers have been shown to facilitate cell migration in the direction of fiber alignment while oxygen (O2)-carrying solutions improve the metabolism of cells in hypoxic culture. Therefore, U251 aggregate migration on poly(ε-caprolactone) (PCL)-aligned fibers was studied in cell culture media supplemented with the O2 storage and transport protein hemoglobin (Hb) obtained from bovine, earthworm and human sources at concentrations ranging from 0 to 5 g/L within a cell culture incubator exposed to O2 tensions ranging from 1 to 19% O2. Individual cell migration was quantified using a wound healing assay. In addition, U251 cell aggregates were developed and aggregate dispersion/cell migration quantified on PCL-aligned fibers. The results of this work show that the presence of bovine or earthworm Hb improved individual cell viability at 1% O2, while human Hb adversely affected cell viability at increasing Hb concentrations and decreasing O2 levels. The control data suggests that decreasing the O2 tension in the incubator from 5 to 1% O2 decreased aggregate dispersion on the PCL-aligned fibers. However, the addition of bovine Hb at 5% O2 significantly improved aggregate dispersion. At 19% O2, Hb did not impact aggregate dispersion. Also at 1% O2, aggregate dispersion appeared to increase in the presence of earthworm Hb, but only at the latter time points. Taken together, these results show that Hb-based O2 carriers can be utilized to improve O2 availability and the migration of glioma spheroids on nanofibers. © 2014 American Institute of Chemical Engineers Biotechnol. Prog., 30:1214-1220, 2014
- Subjects
HEMOGLOBINS; CELL migration; GLIOMAS; NANOFIBERS; CELL metabolism; HYPOXEMIA; PROTEIN transport
- Publication
Biotechnology Progress, 2014, Vol 30, Issue 5, p1214
- ISSN
8756-7938
- Publication type
Article
- DOI
10.1002/btpr.1950