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- Title
HCG + ACTH stimulation of in vitro dehydroepiandrosterone production in human fetal adrenals from precursor cholesterol and Δ<sup>5</sup>-pregnenolone.
- Authors
Lehmann, W. D.; Lauritzen, C.
- Abstract
Fetal adrenal glands were obtained from legal abortions in the 14-22 gestational weeks. The adrenal cortex was separated from the medulla using a micromanipulator. The cortex was homogenized in a glass-teflon homogenizer and the microsomal fraction was isolated by centrifugation. Tissue slices were also prepared by the method of DEUTSCH. The microsomal fraction or the slices were incubated with (4-14C) pregnenolone or (4-14C) cholesterol in the presence of an NADPH regenerating System and oxygen. ACTH or HCG was added. After extraction and paperchromatography radioactivity was determined in a scanner. The conversion of pregnenolone to 17 α-hydroxypregnenolone and dehydroepiandrosterone was increased by ACTH. In the presence of HCG dehydroepiandrosterone production only was increased, - and this occurred in slices only. In the microsomal fraction HCG was without effect on steroid biogenesis from dehydroepiandrosterone.
- Publication
Journal of Perinatal Medicine, 1975, Vol 3, Issue 4, p231
- ISSN
0300-5577
- Publication type
Article