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- Title
Variables pre-analíticas que afectan las concentraciones de homocisteína: Aplicación para biobancos con fines de investigación.
- Authors
Serrano Díaz, Norma C.; Páez Leal, María Carolina; Bautista Niño, Paula Katherine; Díaz-Martínez, Luis Alfonso; Guío Mahecha, Elizabeth
- Abstract
Objective: This study evaluated the impact of pre-analytical variables on serum concentrations of homocysteine, and its possible applications in biobanks for research purposes. Methods: Peripheral blood samples from ten self-reported healthy volunteers were obtained under different conditions of fasting and body position (supine and sitting). Blood samples were temporarily stored for different time intervals between its collection and its centrifugation. All aliquots were stored at -80°C in our biobank until processing. Duplicate Homocysteine measurements were performed in Immulite® 2000. A concordance analysis was performed using Lin coefficient (a) and MANOVA. Results: The measurement of homocysteine is highly reproducible (σ = 0.908, 95% CI 0861-0955) and fasting or time before centrifugation does not affect its concentration. However, we found an average reduction of 14.2% (95% CI 8.4% to 20.0%) for the Hcy levels in samples obtained when the subjects were in supine position as compared to the levels in sitting position. Conclusion: Homocysteine levels are stable, when the blood samples remain without centrifugation, up to 24 hours at 4°C. However, body position of the participant at the time of sampling can affect Hcy levels. These findings highlight the role of biobanks in standardizing, handling and storing biological samples with excellence criteria.
- Subjects
HOMOCYSTEINE; HOMOCYSTEINE in the body; BIOBANKS; QUALITY control; MEDICAL research
- Publication
MedUNAB, 2013, Vol 16, Issue 2, p59
- ISSN
0123-7047
- Publication type
Article
- DOI
10.29375/01237047.1843