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- Title
The expression and cellular localization of phospholipase D isozymes in the developing mouse testis.
- Authors
Seungjoon Kim; Heechul Kim; Yongduk Lee; Jin Won Hyun; Young Ho Lee; Min Kyoung Shin; Do Sik Min; Taekyun Shin
- Abstract
To examine the involvement of phospholipase D (PLD) isozymes in postnatal testis development, the expression of PLD1 and PLD2 was examined in the mouse testis at postnatal weeks 1, 2, 4, and 8 using Western blot analysis and immunohistochemistry. The expression of both PLD1 and PLD2 increased gradually with development from postnatal week 1 to 8. Immunohistochemically, PLD immunoreactivity was detected in some germ cells in the testis and interstitial Leydig cells at postnatal week 1. PLD was mainly detected in the spermatocytes and residual bodies of spermatids in the testis after 8 weeks after birth. The intense immunostaining of PLD in Leydig cells remained unchanged by postnatal week 8. These findings suggest that PLD isozymes are involved in the spermatogenesis of the mouse testis.
- Subjects
PHOSPHOLIPASES; ISOENZYMES; TESTIS; WESTERN immunoblotting; IMMUNOHISTOCHEMISTRY; ANIMAL models in research
- Publication
Journal of Veterinary Science, 2007, Vol 8, Issue 3, p209
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2007.8.3.209