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- Title
Comparative Analysis of Motility Characteristics and Kinematic Parameters of Fresh, Chilled and Sexed Ram Semen - Preliminary study.
- Authors
Yotov, Stanimir; Kistanova, Elena; Abadjieva, Desislava; Karadaev, Manol; Ivanova, Boyana; Bonev, George; Yarkov, Dobri; Sinapov, Branimir
- Abstract
The application of the bovine serum albumin (BSA) column method for sexing of spermatozoa is cost effective and appropriate for small ruminants. The current study compares motility characteristics and kinematic parameters of fresh, chilled and sexed ram semen with the aim to select the high-quality ejaculates for semen sexing. Fresh, chilled and sexed semen from 4 East Friesian rams was analysed by CASA, and immotile sperm cells, motile sperm cells, progressive motility, non-progressive motility, VCL, VAP, VSL, STR, LIN and WOB were determined. Semen sexing was carried out in bovine serum albumin columns and incubation for 45 min at a temperature of 25°C. The average values of all semen parameters and the change of the most important indices in individual rams were estimated. Significant differences (P < 0.05) were detected between immotile and motile sperm cells, progressive and non-progressive motility, VCL and WOB of fresh and chilled semen. All investigated parameters between fresh and isolated upper and bottom layer spermatozoa, excluding STR and WOB, differed significantly (P < 0.05). The same dependency (P < 0.05) was detected for motile sperm, progressive motility, VCL, STR and LIN between chilled and sexed spermatozoa. In conclusion, the average values for motile sperm, progressive motility, VCL, STR and LIN of chilled and sexed ram spermatozoa are significantly (P < 0.05) affected by chilling and sexing in BSA columns at 25°C for 45 min. CASA analysis of motility and kinematic parameters of chilled semen can provide a correct choice of ejaculates with high quality for sexing. The individual features of rams have influence on the semen characteristics and should be take into consideration in selection of the donors for a production of the sexed semen.
- Subjects
SPERM motility; SERUM albumin; RUMINANTS; SPERMATOZOA; KINEMATICS
- Publication
Veterinarija ir Zootechnika, 2021, Vol 79, Issue 2, p37
- ISSN
1392-2130
- Publication type
Article