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- Title
Structure of the ribosome-bound cricket paralysis virus IRES RNA.
- Authors
Schüler, Martin; Connell, Sean R.; Lescoute, Aurelie; Giesebrecht, Jan; Dabrowski, Marylena; Schroeer, Birgit; Mielke, Thorsten; Penczek, Pawel A.; Westhof, Eric; Spahn, Christian M. T.
- Abstract
Internal ribosome entry sites (IRESs) facilitate an alternative, end-independent pathway of translation initiation. A particular family of dicistroviral IRESs can assemble elongation-competent 80S ribosomal complexes in the absence of canonical initiation factors and initiator transfer RNA. We present here a cryo-EM reconstruction of a dicistroviral IRES bound to the 80S ribosome. The resolution of the cryo-EM reconstruction, in the subnanometer range, allowed the molecular structure of the complete IRES in its active, ribosome-bound state to be solved. The structure, harboring three pseudoknot-containing domains, each with a specific functional role, shows how defined elements of the IRES emerge from a compactly folded core and interact with the key ribosomal components that form the A, P and E sites, where tRNAs normally bind. Our results exemplify the molecular strategy for recruitment of an IRES and reveal the dynamic features necessary for internal initiation.
- Subjects
RIBOSOMES; ORGANELLES; RNA; NUCLEIC acids; MOLECULAR biology; BIOMOLECULES
- Publication
Nature Structural & Molecular Biology, 2006, Vol 13, Issue 12, p1092
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb1177