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- Title
Mutation analysis ofB-RAFgene in human gliomas.
- Authors
Basto, Diana; Trovisco, Vítor; Lopes, José M.; Martins, Albino; Pardal, Fernando; Soares, Paula; Reis, Rui M.
- Abstract
The RAS/RAF/MEK/ERK kinase pathway is pivotal in the transduction of mitogenic stimuli from activated growth factor receptors, which regulates cell proliferation, survival, and differentiation. Up-regulation of this pathway due toRASmutations is found in approximately 30% of human tumors. Recently, activating mutations ofB-RAFwere identified in a large proportion of human cancers. Gliomas are the most frequent primary central nervous system tumors and the molecular mechanisms that underlie the development and progression of these tumors are far from being completely understood. The purpose of this study was to clarify the incidence ofB-RAFmutations and their possible relation with tumor progression in a series of 82 human gliomas, including 49 astrocytic and 33 oligodendroglial tumors. The analysis ofB-RAFhotspot regions, exons 11 and 15, showed presence ofB-RAFmutations in only 2 out of 34 (6%) glioblastomas, and absence in the remaining histological types. Both mutations were located in the hotspot residue 600 (V600E) at exon 15, which leads to constitutive B-RAF kinase activity. These data suggest that activating mutations ofB-RAFare not a frequent event in gliomas; nevertheless, when present they are associated with high-grade malignant lesions.
- Subjects
CELL proliferation; GENETIC mutation; GENES; GLIOMAS; NERVOUS system tumors; CELL growth
- Publication
Acta Neuropathologica, 2005, Vol 109, Issue 2, p207
- ISSN
0001-6322
- Publication type
Article
- DOI
10.1007/s00401-004-0936-x